黄自坤

姓名：黄自坤

职务\职称：副主任技师、科副主任

系别：临床检验诊断学

研究方向：抗感染免疫、自身免疫

工作邮箱：yfyhzk@163.com

办公电话：0791-88693136

简介：毕业于南昌大学，副主任技师，现任南昌大学第一附属医院检验科副主任，硕士生导师。为中华医学会医学细胞生物学分会青年委员，中国麻风防治协会皮肤性病检验与诊断分会青年委员，江西省医学会微生物学与免疫学分会青年委员会副主任委员，江西省研究型医院学会检验分会青年委员会副主任委员。主要研究方向为抗感染免疫及自身免疫，近年先后入选江西省“双千计划”、江西省杰出青年人才资助计划、江西省“远航工程”，为南昌大学第一附属医院青年学科带头人培养对象。主持国家自然科学基金课题2项、省级课题4项、厅级课题4项。以第一作者或通讯作者发表论文60余篇，其中SCI收录33篇；申请国家发明专利2项；获江西省科技进步二等奖1项。

论文、专著与专利：

1.Zikun Huang, Fangyi Yao, Jun Liu, Jianqing Xu, Yang Guo, Rigu Su, Qing Luo*, Junming Li*. Up-regulation of circRNA-0003528 promotes mycobacterium tuberculosis associated macrophage polarization via down-regulating miR-224-5p, miR-324-5p and miR-488-5p and up-regulating CTLA4. Aging, 2020.09 (Accepting).

2. Zikun Huang, Jianing Liu, Lu. Li, Yang Guo, Qing Luo*, Junming Li*. Long non-coding RNA expression profiling of macrophage line RAW264.7 infected by Mycobacterium tuberculosis. Biotech Histochem, 2020, 95(6):403-410.

3. Zikun Huang, Jinghui Du, Zhen Deng, Qing Luo, Guoliang Xiong, Yaoxing Wang, Xi Zhang, Junming Li*. Multicentre laboratory validation of the nitrate reductase assay using liquid medium for the rapid detection of multidrug-resistant and extensively drug-resistant Mycobacterium tuberculosis. Tuberculosis, 2018, 113:242-248.

4.Zikun Huang, Rigu Su, Cheng Qing, Yiping Peng, Qing Luo*, Junming Li*. Plasma circular RNAs hsa_circ_0001953 and hsa_circ_0009024 as diagnostic biomarkers for active tuberculosis. Front Microbiol, 2018, 9:2010.

5.Zikun Huang, Rigu Su, Fangyi Yao, Yiping Peng, Qing Luo*, Junming Li*. Circulating circular RNAs hsa_circ_0001204 and hsa_circ_0001747 act as diagnostic biomarkers for active tuberculosis detection. Int J Clin Exp Pathol, 2018, 11(2): 586-594.

6.Zikun Huang, Fangyi Yao, Jianqing Xu, Zhen Deng, Rigu Su, Yiping Peng, Qing Luo*, Junming Li*. Microarray Expression Profile of Circular RNAs in Peripheral Blood Mononuclear Cells from Active Tuberculosis Patients. Cell Physiol Biochem, 2018, 45: 1230-1240.

7.Zikun Huang, Rigu Su, Zhen Deng, Jianqing Xu, Yiping Peng, Qing Luo*, Junming Li*. Identification of differentially expressed circular RNAs in human monocyte derived macrophages response to Mycobacterium tuberculosis infection. Sci Rep, 2017, 7(1):13673.

8.Zikun Huang, Fangyi Yao, Zhen Deng, Surigu, Yiping Peng, Qing Luo*, Junming Li*. Elevated expression of Tim-3 on CD56dimCD16+ NK cells correlates with disease severity of pulmonary tuberculosis. Int J Clin Exp Pathol, 2017, 10(5):5031-5038.

9.Zikun Huang, Qing Luo, Fangyi Yao, Cheng Qing, Jianqing Ye, Yating Deng, Junming Li*, Identification of Differentially Expressed Long Non-coding RNAs in Polarized Macrophages. Sci Rep, 2016, 6:19705.

10. Zikun Huang, Qing Luo, Cheng Qing, Yating Deng, Guoliang Xiong, Junming Li*. Evaluation of the direct nitrate reductase assay for rapid detection of extensively drug-resistant tuberculosis. Int J Tuberc Lung Dis, 2016, 20(4):468-473.

11.Zikun Huang, Cheng Qin, Jinghui Du, Qing Luo, Yaoxing Wang, Wang Zhang, Xi Zhang, Guoliang Xiong, Jie Cheng, Xiaomeng Xu, Weiting Li, Junming Li*. Evaluation of the microscopic observation drug susceptibility assay for the rapid detection of MDR-TB and XDR-TB in China: a prospective multicentre study. J Antimicrob Chemother, 2015, 70(2): 456-462.

12.Zikun Huang, Qing Luo, Yang Guo, Jie Chen, Guoliang Xiong, Yiping Peng, Jianqing Ye, Junming Li*. Mycobacterium tuberculosis-Induced Polarization of Human Macrophage Orchestrates the Formation and Development of Tuberculous Granulomas In Vitro. PLoS One, 2015, 10(6):e0129744.

13.Zikun Huang, Guangming Li, Jie Cheng, Weiting Li, Xiaomeng Xu, Qing Luo, Guoliang Xiong, Jian Sun, Junming Li*. Evaluation of MODS assay for rapid detection of Mycobacterium tuberculosis resistance to second-line drugs in a tertiary care tuberculosis hospital in China. Tuberculosis, 2014, 94(5): 506-510.

14.Zikun Huang, Guoliang Xiong, Qing Luo, Bixia Jiang, Weiting Li, Xiaomeng Xu, Junming Li*. Evaluation of the performance of the microscopic observation drug susceptibility assay for diagnosis of extrapulmonary tuberculosis in China: A preliminary study. Respirology, 2014, 19(1): 132-137.

15.Zikun Huang, Qing Luo, Guoliang Xiong, Weiting Li, Xiaomeng Xu, Junming Li*, Evaluation of indirect drug susceptibility testing using the MODS assay for the detection of XDR-TB in China. Int J Tuberc Lung Dis, 2014, 18(4): 461-465.

16. Qing Luo, Biqi Fu, Lu Zhang, Yang Guo, Zikun Huang*, Junming Li*. Expression and clinical significance of circular RNA hsa_circ_0079787 in the peripheral blood of patients with axial spondyloarthritis. Mol Med Rep, 2020, 22(5):4197-4206.

17. Qing Luo, Lu Zhang, Lihong Xiong, Biqi Fu, Yang Guo, Zikun Huang*, Junming Li*. Peripheral blood circular RNA hsa_circ_0082688-hsa_circ_0008675 can be used as a candidate biomarker of systemic lupus erythematosus with renal involvement. Clin Exp Rheumatol, 2020 Sep 16. Online ahead of print.

18. Qing Luo, Yujie Gao, Lu Zhang, Jiayue Rao, Yang Guo, Zikun Huang*, Junming Li*. Decreased ALKBH5, FTO, and YTHDF2 in Peripheral Blood Are as Risk Factors for Rheumatoid Arthritis. Biomed Res Int, 2020, 2020:5735279.

19. Qing Luo, Biqi Fu, Lu Zhang, Yang Guo, Zikun Huang*, Junming Li*. Decreased Peripheral Blood ALKBH5 Correlates with Markers of Autoimmune Response in Systemic Lupus Erythematosus. Dis Markers, 2020, 2020:8193895.

20. Qing Luo, Jiayue Rao, Lu Zhang, Biqi Fu, Yang Guo, Zikun Huang*, Junming Li*. The study of METTL14, ALKBH5, and YTHDF2 in peripheral blood mononuclear cells from systemic lupus erythematosus. Mol Genet Genomic Med, 2020, 8(9):e1298.

21. Qing Luo, Lu Zhang, Le Fang, Biqi Fu, Yang Guo, Zikun Huang*, Junming Li*. Circular RNAs hsa_circ_0000479 in peripheral blood mononuclear cells as novel biomarkers for systemic lupus erythematosus. Autoimmunity, 2020, 53(3):167-176.

22. Qing Luo, Lulu Zeng, Lingbing Zeng, Jiayue Rao, Lu Zhang, Yang Guo, Zikun Huang*, Junming Li*. Expression and clinical significance of circular RNAs hsa_circ_0000175 and hsa_circ_0008410 in peripheral blood mononuclear cells from patients with rheumatoid arthritis. Int J Mol Med, 2020, 45(4):1203-1212.

主持的科研项目：

1.2021.01-2024.12，国家自然科学基金项目（32060181），“tRF-3027b通过SIRT6调节巨噬细胞自噬功能参与结核菌感染的机制研究”，36万元

2.2020.01-2022.12，江西省双千计划青年项目，100万元

3.2018.01-2020.12，江西省科技厅重点研发计划项目（20181BBG70012），“结核病活化相关中性粒细胞差异蛋白的筛选及其在结核病诊断中的应用”，20万元

4. 2018.01-2020.12，江西省教育厅科技计划项目（GJJ170001），“结核菌感染诱导巨噬细胞极化相关环状RNA分子的筛选及其调控机制研究”，5万元

5. 2019.01-2020.12，江西卫健委科技计划项目（20191052），“血浆环状RNA作为生物标志物在肺结核病早期诊断中的应用研究”，0.4万元

6. 2016.01-2019.12，国家自然科学基金项目（81560001），“结核菌感染过程中LncRNA-AK140703对巨噬细胞极化改变的影响及其调控机制研究”，35万元

7. 2017.01-2019.12，江西省杰出青年人才资助计划项目（20171BCB23087），15万元

8. 2017.01-2019.12，江西省自然科学基金项目（20171BAB205111），“长链非编码RNA-AK017011抑制自噬促进结核菌感染免疫逃逸的机制研究”，6万元

9. 2015.08-2018.08，江西省科技支撑计划项目（20151BBG70208），“血清长链非编码RNA作为肺结核病早期诊断新分子标志物的筛选与鉴定”，4万元

10. 2014.01-2016.12，江西省青年科学基金项目（20142BAB215057），“负性共刺激分子B7H1在中性粒细胞抗结核免疫中的作用及其调控机制”，5万元